scd59 (Sino Biological)
Sino Biological is a verified supplier 
Sino Biological manufactures this product 
93
Structured Review
Sino Biological
scd59
Scd59, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scd59/CD59+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pmc10550724-328-37-40
Average 93 stars, based on 3 article reviews
Scd59, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scd59/CD59+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pmc10550724-328-37-40
Average 93 stars, based on 3 article reviews
scd59 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration"
Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration
Journal: iScience
doi: 10.1016/j.isci.2023.107939
Figure Legend Snippet: In vivo expression of eGFP, PEDF, sFlt-1, and sCD59 following IVT administration of AdCs (A) Representative fundus images of the retina from individual mice following IVT injection of 1.5×10 9 vp and 7.5×10 9 vp AdC68-eGFP. The eGFP signal could be detected from 48 h to 35 days post-injection. The dotted circles represent the edge of mouse retina. (B–D) Assessment of PEDF, sFlt-1, and sCD59 mRNA expression in retina-choroid-sclera complexes isolated from 10 mice. In each mouse, one eye was injected with AdC68-PFC (five mice for 1.5×10 9 vp and five mice for 7.5×10 9 vp) whereas the contralateral, un-injected eye served as control (only five eyes were used for analysis). At 4 days post-injection, RNA was purified from the retina-choroid complexes and real-time qPCR was conducted. Absolute number of mRNA copies were calculated using the standard curve method. (E–H) Images of western blot and quantification of the PEDF, sFlt-1, and sCD59 protein amount expressed in retina-choroid complexes of five mice. In each mouse, one eye was injected with AdC68-PFC (7.5×10 9 vp) whereas the contralateral, un-injected eye served as control. Total protein was obtained from retina-choroid-sclera complexes isolated from AdC68-PFC-treated (7.5×10 9 vp) and un-injected eyes 7 days post-injection. Antibodies against GAPDH were used for the internal control. The relative expression of PEDF, sFlt-1, and sCD59 in the un-injected eyes was set to 1. Data are expressed as mean ± SEM, and analyzed using one-way ANOVA multiple comparisons with Tukey’s method among groups in (B) and Student’s t test (two-tailed) in (C) (∗p < 0.05, ∗∗p < 0.01). PEDF, pigment epithelium-derived factor; sFlt-1, soluble fms-like tyrosine kinase-1; sCD59, soluble forms of CD59; IVT, intravitreal.
Techniques Used: In Vivo, Expressing, Injection, Isolation, Control, Purification, Western Blot, Two Tailed Test, Derivative Assay
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Transfection, Protease Inhibitor, Plasmid Preparation, Gel Extraction, Software
Related Articles
In Vivo:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Expressing:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Injection:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Isolation:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Control:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Purification:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Western Blot:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Two Tailed Test:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Derivative Assay:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Virus:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Recombinant:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Transfection:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Protease Inhibitor:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Plasmid Preparation:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Gel Extraction:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), Software:Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration Article Snippet: loaded and electrophoresed in 8–15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (IPVH00010, Millipore, USA). .. PVDF membranes were blocked with 5% milk in PBST (PBS+Tween-20) for 2 h at RT and then target proteins were detected by specific primary antibodies including PEDF (1:500, 11104-RP02, Sino Biological, China), VEGFR1 (1:500, AF7748, Affinity, US), |
